IL12A Rabbit Polyclonal Antibody

• Catalog Number: orb10893
• Category: Antibodies
• Description: IL12A Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse IL-12 (51-150/215aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 22 kDa
• Target: IL12A
• RRID: AB_10750980
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Interleukin-12 subunit alpha; IL-12; CLMF p35; CLM
• Background: _CLIENT_OVERRIDE_
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Yujie Yang 1, Shen Zhang 1, Jin Yang 1, Changheng Yao 1, Xue Li 1, Wenling Dai 2, Jihua Liu The Aqueous Extract of Armadillidium vulgare Latreille Alleviates Neuropathic Pain via Inhibiting Neuron-Astrocyte Crosstalk Mediated by the IL-12-IFN-γ-IFNGR-CXCL10 Pathway J Ethnopharmacol ., (2024)

PubMed: 39617087

Blank control (blue line): Mouse spleen (blue). Primary Antibody (green line): Rabbit Anti-IL12 antibody (orb10893), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ice-cold methanol overnight at 4°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (orb10893) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (orb10893) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: Blood cell (Mouse) Lysate at 40 ug, Lane 3: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 4: Liver (Mouse) Lysate at 40 ug, Lane 5: Spleen (Rat) Lysate at 40 ug, Lane 6: Liver (Rat) Lysate at 40 ug, Primary: Anti-IL12 (orb10893) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 35 kD.

Sample: Liver (Mouse) Lysate at 40 ug, Spleen (Mouse) Lysate at 40 ug, NIH/3T3 (Mouse) CellLysate at 30 ug, RAW246.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-IL12 (orb10893) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 35/36 kD.