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IL1 Receptor antagonist antibody
Description
Research Area
Images & Validation
−| Tested Applications | ICC, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IL1 Receptor antagonist. The exact sequence is proprietary. |
| Target | IL1 Receptor antagonist |
| Molecular Weight | 20 |
| Purity | Purified by antigen-affinity chromatography. |
| Purification | Purified by antigen-affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Buffer/Preservatives | 0.025% ProClin 300. 1XPBS (pH7), 20% Glycerol |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−IL-1RA Rabbit Polyclonal Antibody [orb6227]
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Human
Rabbit
Polyclonal
Unconjugated
100 μl, 200 μl, 50 μlIL1RN Antibody [orb2309957]
IHC
Human
Mouse
Monoclonal
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100 μg (without BSA and Azide), 100 μg, 20 μgDog Interleukin 1 Receptor Antagonist (IL1RA) ELISA Kit [orb779742]
Canine
31.25-2000 pg/mL
12.7 pg/mL
96 T, 48 T

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Immunofluorescence analysis of HeLa cells using IL1 Receptor antagonist antibody

Immunohistochemical staining of PC3 xenograft using IL1 Receptor antagonist antibody

Western blot analysis of PC-12 whole cell lysate using IL1 Receptor antagonist antibody

Western blot analysis of Neuro2A whole lysate(Lane 1), C8D30 whole cell lysate(Lane 2), NIH-3T3 whole cell lysate(Lane 3), Raw264.7 whole cell lysate(Lane 4), C2C12 whole cell lysate(Lane 5) using IL1 Receptor antagonist antibody

Western blot analysis of Raji cells using IL1 Receptor antagonist antibody
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Protocol Information
IL1 Receptor antagonist antibody (orb556363)
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