IL1RN Antibody (C-term)

• Catalog Number: orb1930039
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18902
• Tested applications: FC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:4000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 20055 Da
• Target: This IL1RN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-177 amino acids from the C-terminal region of human IL1RN.
• UniProt ID: P18510
• NCBI: NP_776214.1, NP_776215.1, NP_000568.1, NP_776213.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Interleukin-1 receptor antagonist protein, IL-1RN,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IL1RN Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of IL1RN Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

IL1RN Antibody (C-term) western blot analysis in 293, Hela cell line lysates (35 ug/lane). This demonstrates the IL1RN antibody detected the IL1RN protein (arrow).

Anti-IL1RN Antibody (C-term) at 1:4000 dilution + 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.