IL-1RA Rabbit Polyclonal Antibody

• Catalog Number: orb6227
• Category: Antibodies
• Description: IL-1RA Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse IL-1RA (26-120/178aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17 kDa
• Target: IL1RN
• UniProt ID: P25085
• RRID: AB_10950603
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Interleukin-1 receptor antagonist; ICIL 1RA; IL1 i
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Dutta, Puja et al. Neutralization of TNF-α and IL-1β Regulates CXCL8 Production through CXCL8/CXCR1 Axis in Macrophages during Staphylococcus aureus Infection Immunol. Invest., (2020)

PubMed: 32602757

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL-1RA) Polyclonal Antibody, Unconjugated (orb6227) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Plasma (Mouse) Tissue Lysate at 30 ug, Lane 2: Hemocyte (Mouse) Tissue Lysate at 30 ug, Primary: Anti-IL-1RA (orb6227) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kD, Observed band size: 23 kD.

Sample: Spleen (Mouse) Tissue Lysate at 30 ug, Primary: Anti-IL-1RA (orb6227) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kD, Observed band size: 23 kD.

Tissue/Cell: Human oral squamous cell carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-1RA Polyclonal Antibody, Unconjugated (orb6227) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.