IL-17A antibody

• Catalog Number: orb345132
• Category: Antibodies
• Description: IL-17A antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: IL-17A Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-A protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1:000 - 1:5,000, IHC: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-IL-17A Antibody was affinity purified from monospecific antiserum by Protein A Purification. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. Cross-reactivity with IL-17 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q16552
• NCBI: AAH662531.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: rabbit anti-Interleukin-17A antibody, rabbit anti-
• Note: For research use only
• Application notes: IL-17-A antibody has been tested for use in IHC and western blotting and is suitable in ELISA. By western blot a band approximately ~17.5 kDa in size corresponding to human IL-17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1