IL-17A Antibody

• Catalog Number: orb1272699
• Category: Antibodies
• Description: IL-17A Antibody
• Target: Il17a
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-17A (murine IL-17A). Murine IL-17A specific antibody was purified by affinity chromatography employing immobilized mIL-17A matrix.
• UniProt ID: Q62386
• Tested applications: ELISA, WB
• Application notes: ELISA:Indirect:To detect mIL-17A by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A.SandwichTo detect mIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-17A as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A. Western Blot:To detect mIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Il17, Ctla8, IL-17, Ctla-8, IL-17A, Il17, Interleu
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: Q62386

To detect mIL-17A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-17A (orb1272698) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A.

To detect mIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.