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Catalog Number | orb1272479 |
---|---|
Category | Antibodies |
Description | IL-17 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Human |
Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-17 (human Interleukin-17). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | IL17A |
UniProt ID | Q16552 |
NCBI | Q16552 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Read more... |
Note | For research use only |
Application notes | Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-17A (50.00 ng/mL), a concentration of 0.43 - 0.65 μg/mL of this antibody is required.ELISA:To detect hIL-17A by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A. Sandwich:To detect hIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-17A (XP-5171Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.Western Blot:To detect hIL-17 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect hIL-17A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human IL-17A (orb1272478) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.
To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 ug/ml - 0.25 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
ELISA, IF, IHC-P, WB | |
Bovine, Porcine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Rat | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |