IL-17 Antibody

• Catalog Number: orb1239678
• Category: Antibodies
• Description: IL-17 Antibody
• Target: IL17A
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: IL-17 Antibody is affinity chromatography purified via peptide column.
• Immunogen: IL-17 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-17A.The immunogen is located within amino acids 40 - 90 of IL-17.
• UniProt ID: Q16552
• MW: Predicted: 17.5kDObserved: 20 kD (Post-modification: 1 N-linked glycosylation)
• Tested applications: ELISA, WB
• Application notes: WB: 0.125-4 μg/mL.Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17,
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_002181

Western Blot Validation in Human THP-1 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-17 orb1239678 (A: 1 µg/mL and B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in A-20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-17 orb1239678 (A: 2 µg/mL and B: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Recombinant Protein Test. Loading: 30 ng of human IL-17 recombinant protein per lane. Antibodies: IL-17 orb1239678, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.125 µg/mL, Lane 2: 0.25 µg/mL, Lane 3: 0.5 µg/mL.