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IGF2BP1 Antibody

SKU: orb1249100

Description

IGF2BP1 Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5kDa according to NP_006537.3). Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 3.75µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-EKVFAEHKISYSGQ
TargetIGF2BP1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

IGF2BP1, insulin-like growth factor 2 mRNA binding protein 1, CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1, IGF II mRNA binding protein 1, IGF2 mRNA-binding protein 1, VICKZ family member 1, coding region determinant-binding protein, zipcode-binding protein 1

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IGF2BP1 Antibody

orb1249100 (0.3 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

IGF2BP1 Antibody

orb1249100 (3.75 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IGF2BP1 Antibody (orb1249100)

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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