IGF2BP1 Antibody

• Catalog Number: orb1249100
• Category: Antibodies
• Description: IGF2BP1 Antibody
• Target: IGF2BP1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: The immunogen for this antibody is: C-EKVFAEHKISYSGQ
• UniProt ID: Q9NZI8
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5kDa according to NP_006537.3). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 3.75ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IGF2BP1, insulin-like growth factor 2 mRNA binding
• Note: For research use only
• NCBI: NP_001153895.1, NP_006537.3

orb1249100 (0.3 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249100 (3.75 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.