IDH3A Antibody

• Catalog Number: orb1259852
• Category: Antibodies
• Description: IDH3A Antibody
• Target: IDH3A
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-366 of human IDH3A (NP_005521.1).
• UniProt ID: P50213
• MW: Observed: 40kDa
• Tested applications: IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IDH3A,
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only

Western blot analysis of extracts of various cell lines, using IDH3A antibody (orb1259852) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat kidney using IDH3A antibody (orb1259852) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human thyroid cancer using IDH3A antibody (orb1259852) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH3A antibody (orb1259852) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using IDH3A antibody (orb1259852) at dilution of 1:100 (40x lens).