IDH3A Antibody

• Catalog Number: orb22494
• Category: Antibodies
• Description: Goat polyclonal antibody to IDH3A
• Target: IDH3A
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DFTEEICRRVKDLD
• RRID: AB_10955331
• MW: 39.6
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: WB: Approx 37kDa band observed in lysates of Human Lymph Nodes, and of cell line DAUDI (calculated MW of 39.6kDa according to NP_005521.1). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti IDH3A antibody, anti isocitrate dehydrogenase
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 3419

orb22494 (0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of staining of Human (A), Mouse (B) and Rat (C) Spleen lysates using IDH3A antibody.

Western blot analysis of staining of Pig Spleen (A) and Pig Skeletal Muscle (B) lysates using IDH3A antibody.

Western blot analysis of Human Lymph Nodes lysate (35ug protein in RIPA buffer) using IDH3A antibody