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Catalog Number | orb22494 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to IDH3A |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml |
Conjugation | Unconjugated |
MW | 39.6 |
Target | IDH3A |
Entrez | 3419 |
Protein Sequence | DFTEEICRRVKDLD |
RRID | AB_10955331 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti IDH3A antibody, anti isocitrate dehydrogenase Read more... |
Note | For research use only |
Application notes | WB: Approx 37kDa band observed in lysates of Human Lymph Nodes, and of cell line DAUDI (calculated MW of 39.6kDa according to NP_005521.1). Recommended concentration: 0.1-0.3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb22494 (0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of staining of Human (A), Mouse (B) and Rat (C) Spleen lysates using IDH3A antibody.
Western blot analysis of staining of Pig Spleen (A) and Pig Skeletal Muscle (B) lysates using IDH3A antibody.
Western blot analysis of Human Lymph Nodes lysate (35ug protein in RIPA buffer) using IDH3A antibody