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Catalog Number | orb392696 |
---|---|
Category | Proteins |
Description | Recombinant human VEGFA protein is produced by our E. coli expression system and the target gene encoding Ala27-Arg147 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | VEGFA |
Entrez | 7422 |
UniProt ID | P15692 |
Protein Sequence | MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | VEGF; Vascular endothelial growth factor A; VEGF-A Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
19.2 kDa | |
Human VEGF165, premium grade (orb257923) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4). |
Unconjugated | |
90% | |
21.1 kDa | |
Human VEGF165, His Tag (orb257924) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4). |
Unconjugated | |
95% | |
14.1 kDa | |
Human VEGF121 Protein, premium grade (orb257918) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 147 (Accession # P15692-9). |
Unconjugated | |
95% | |
14.9 kDa | |
Human VEGF121, His Tag (orb334936) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 147 (Accession # P15692-9). |
Unconjugated | |
95% | |
84.1 kDa | |
Human VEGF R1 Protein, His Tag (orb1184768) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Asn 756 (Accession # P17948-1). |
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