Anti-VEGF/VEGFA Antibody

• Catalog Number: orb196242
• Category: Antibodies
• Description: Anti-VEGF/VEGFA Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Immunogen: E.coli-derived human VEGF recombinant protein (Position: A27-R191). Human VEGF shares 78% amino acid (aa) sequence identity with both mouse and rat VEGF.
• UniProt ID: P15692
• MW: 18, 27 kDa
• Tested applications: FC, IHC, WB
• Application notes: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Vascular endothelial growth factor A; VEGF-A; Vasc
• Note: For research use only

Flow Cytometry analysis of SiHa cells using anti-VEGF antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of VEGF using anti-VEGF antibody.Lane 1:rat lung Tissue;2:rat lver Tissue;3:rat thymus Tissue;4:mouse lung Tissue;5:mouse thymus Tissue;6:HEPA1-6 Cell;7:human placenta Tissue;8:A431 Cell.

IHC analysis of VEGF using anti-VEGF antibody. VEGF was detected in paraffin-embedded section of human rectal cancer tissue.

IHC analysis of VEGF using anti-VEGF antibody. VEGF was detected in paraffin-embedded section of human rectal cancer tissue.

IHC analysis of VEGF using anti-VEGF antibody. VEGF was detected in paraffin-embedded section of rat brain tissue.