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VEGF antibody
Description
Images & Validation
−| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | IHC-P: 5 μg/ml, WB: 1μg/ml |
| Reactivity | Human, Porcine, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | 4H4G2 |
| Immunogen | Human VEGFA recombinant protein. Protein available as (orb178366) was used as immunogen. |
| Target | VEGF |
| Molecular Weight | 27 kDa |
| Purity | Monoclonal antibodies are purified by peptide affinity chromatography |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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WB analysis of protein 100 ng (lane 1), protein 50 ng (lane 2), protein 20 ng (lane 3) tissue using VEGFA antibody (1 ug/ml)

WB analysis of mouse brain (lane 1), mouse liver (lane 2), mouse kidney (lane 3), hela cells (lane 4) tissue using VEGFA antibody (1 ug/ml)

Immunohistochemical staining of paraffin embedded rat colon tissue using VEGFA antibody (5 ug/ml)

Immunohistochemical staining of pig large intestine tissue using anti-VEGFA (5 ug/ml)

IHC-P image of rat colon tissue using anti-VEGFA (5 ug/ml)
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Protocol Information
Bonacina, F. et al. Pentraxin 3 deficiency protects from the metabolic inflammation associated to diet-induced obesity Cardiovasc. Res., (2019)
VEGF antibody (orb256347)
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