VEGF antibody

• Catalog Number: orb256347
• Category: Antibodies
• Description: VEGF antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4H4G2
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Porcine, Rat
• Isotype: IgG
• Immunogen: Human VEGFA recombinant protein. Protein available as (orb178366) was used as immunogen.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: IHC-P: 5 μg/ml, WB: 1μg/ml
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Monoclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 27 kDa
• Target: VEGF
• Entrez: 5788
• UniProt ID: Q00731, P16612, P15692
• NCBI: 15692, 14
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Vascular endothelial growth factor A antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Bonacina, F. et al. Pentraxin 3 deficiency protects from the metabolic inflammation associated to diet-induced obesity Cardiovasc. Res., (2019)

PubMed: 30859179

WB analysis of protein 100 ng (lane 1), protein 50 ng (lane 2), protein 20 ng (lane 3) tissue using VEGFA antibody (1 ug/ml)

WB analysis of mouse brain (lane 1), mouse liver (lane 2), mouse kidney (lane 3), hela cells (lane 4) tissue using VEGFA antibody (1 ug/ml)

Immunohistochemical staining of paraffin embedded rat colon tissue using VEGFA antibody (5 ug/ml)

Immunohistochemical staining of pig large intestine tissue using anti-VEGFA (5 ug/ml)

IHC-P image of rat colon tissue using anti-VEGFA (5 ug/ml)