VEGFA Human Monoclonal Antibody

SKU: orb2960366

Description

VEGFA Human Monoclonal Antibody is an unconjugated monoclonal antibody. It shows reactivity with Human samples. It is suitable for use in ELISA, FC, NeA applications. The antibody is supplied in liquid form. It is Protein A/G purified from cell culture supernatant. Purity is >95% as determined by SDS-PAGE.

Research Area

Cardiovascular Research; Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, NeA
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: BKX2293
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A

Flow-cytometry using anti-human VEGFA antibody. VEGFA Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # orb2960366, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using APC anti-human VEGFA antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human VEGFA monoclonal antibody (Catalog: orb2960366, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human VEGFA antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGFA monoclonal antibody (Catalog: orb2960366, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human VEGFA.

Quick Database Links

UniProt

P15692