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Catalog Number | orb392694 |
---|---|
Category | Proteins |
Description | Recombinant human VEGFA protein is produced by our mammalian expression system and the target gene encoding Ala27-Arg191 is expressed. |
Target | VEGFA |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Protein Sequence | APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
UniProt ID | P15692 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | VEGF; Vascular endothelial growth factor A; VEGF-A Read more... |
Note | For research use only |
Entrez | 7422 |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 14.8 kDa after removal of the signal peptide. | |
Mammalian |
SDS-PAGE | |
Unconjugated | |
> 85% as determined by SDS-PAGE |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide. The apparent molecular mass of VEGF165-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 40.2 kDa after removal of the signal peptide. The apparent molecular mass of VEGF121-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 40.1 kDa after removal of the signal peptide. The apparent molecular mass of VEGFA-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |