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Human IgM (Fc5u) antibody
Catalog Number: orb346650
| Catalog Number | orb346650 |
|---|---|
| Category | Antibodies |
| Description | Human IgM (Fc5u) antibody |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 22G1.H8 |
| Tested applications | ELISA, WB |
| Reactivity | Human |
| Isotype | IgG2b |
| Immunogen | Anti-Human IgM Fc5µ was produced by repeated immunization with Human IgM Fc5µ fragment in mice. |
| Concentration | 1.0 mg/ml |
| Dilution range | ELISA: 1:1000 - 1:10000, WB: 1:1000-1:2000 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Anti-Human IgM Fc5µ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5µ. No reaction was observed against Human IgG, Human IgA, Human Fc, Human Fab or Human Fab2. |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | mouse anti-Human IgM Fc5µ Antibody, Human IgM Fc5u Read more... |
| Note | For research use only |
| Application notes | Human IgM Fc5µ antibody has been tested by ELISA, SDS-PAGE and Western Blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. |
| Expiration Date | 12 months from date of receipt. |
Human IgM (myeloma) Fc5u Antibody [orb346226]
ELISA, IHC, SDS-PAGE, WB
Unconjugated
Human IgM (myeloma) Fc5µ fragment has been prepared from Human IgM myeloma protein by digestion with trypsin followed by column chromatography. Purity was assessed by SDS-PAGE and HPLC to be greater than 95%. A single precipitin arc was observed against anti-human IgM Fc5µ and anti-human serum when assayed by immuno-electrophoresis at a concentration of 20 mg/ml. No reaction was observed against anti-Trypsin, anti-human IgG F(ab')2, anti-human IgG F(c), anti-human Kappa or anti-human Lambda.
Human
1 mgHuman IgM (myeloma) Fc5u Fluorescein Antibody [orb346236]
FITC
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by trypsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgM Fc5µ and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Trypsin.
Human
1 mgHuman IgM (myeloma) Fc5u Peroxidase Antibody [orb346248]
HRP
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by enzyme digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Human IgM, anti-Human IgM Fc5µ and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2.
Human
1 mgHuman IgM (myeloma) Fc5u Biotin Antibody [orb346259]
Biotin
This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation, ion exchange chromatography followed by tandem molecular sieve chromatography and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti- Human IgM, and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Papain.
Human
1 mgHuman IgM (myeloma) Fc5u Texas Red Antibody [orb346269]
Texas Red
This product was prepared from normal serum by delipidation, selective precipitation and tandem size exclusion chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgM and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Human IgA.
Human
1 mg
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