Human IgM Fc5u Antibody

SKU: orb346650

Description

Human IgM (Fc5u) antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:1000 - 1:10000, WB: 1:1000-1:2000
• Reactivity: Human
• Application Notes:
  Human IgM Fc5µ antibody has been tested by ELISA, SDS-PAGE and Western Blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 22G1.H8
• Immunogen: Anti-Human IgM Fc5µ was produced by repeated immunization with Human IgM Fc5µ fragment in mice.
• Purity: Anti-Human IgM Fc5µ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5µ. No reaction was observed against Human IgG, Human IgA, Human Fc, Human Fab or Human Fab2.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-Human IgM Fc5µ Antibody, Human IgM Fc5u Antibody, Ms-a-Human IgM Fc5µ Antibody, Human IgM Fc5µ Antibody in Mouse, Human M Fc5µ Secondary Antibody

Western Blot of Mouse anti-Human IgM Fc5µ antibody. Lane 1: Human IgM (p/n orb2652752). Lane 2: Human IgG (p/n orb346219). Lane 3: Human Fc (p/n orb346220). Lane 4: Human Fab (p/n orb346222). Lane 5: Human Fab2 (p/n orb346221). Lane 6: Human IgM Fc5µ (p/n orb346226). Load: 50 ng whole molecule per lane. Primary antibody: anti-Human IgM Fc5µ monoclonal antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase anti-mouse secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicated/Observed size: 78 kDa, 78 kDa for Human IgM.