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Description
Images & Validation
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Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:1000 - 1:10000, WB: 1:1000-1:2000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 22G1.H8 |
| Immunogen | Anti-Human IgM Fc5µ was produced by repeated immunization with Human IgM Fc5µ fragment in mice. |
| Purity | Anti-Human IgM Fc5µ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5µ. No reaction was observed against Human IgG, Human IgA, Human Fc, Human Fab or Human Fab2. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−mouse anti-Human IgM Fc5µ Antibody, Human IgM Fc5u Antibody, Ms-a-Human IgM Fc5µ Antibody, Human IgM Fc5µ Antibody in Mouse, Human M Fc5µ Secondary Antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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