F(ab')2 Human IgM Fc5u Antibody Biotin Conjugated

SKU: orb348203

Description

F(ab')2 Human IgM Fc5u antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, FC: 1:200 - 1:1,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
• Reactivity: Human
• Application Notes:
  F(ab')2 Anti-Human IgM Fc5µ Biotin Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. F(ab')2 Anti-Human IgM Fc5µ Biotin Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Human IgM Fc5µ fragment
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgM Fc5µ and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or other human heavy or light chain proteins.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Goat F(ab')2 Anti-Human IgM Fc5µ Antibody Biotin Conjugation, Goat Fab2 Anti-Human IgM Fc5µ Biotin Conjugated Antibody

ELISA Results of F(ab')2 Goat Anti-Human IgM Fc5µ Antibody Biotin Conjugated. Each well was coated in duplicate with 10 µg of Human IgM Fc5µ (p/n orb346226). The working dilution is 297000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3%Fish Gel/PBS, HRP Stabilizer, and TMB substrate (p/n orb348651).