Human IgM (Fc5u) Antibody Alkaline Phosphatase Conjugated

SKU: orb347305

Description

Human IgM (Fc5u) antibody (Alkaline Phosphatase)

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Reactivity: Human
• Application Notes:
  Anti-Human IgM Fc5µ Alkaline Phosphatase conjugate has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Human IgM Fc5µ fragment
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgM and Human Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Human IgM (Fc5u) Antibody alkaline phosphatase Conjugation, Rabbit Anti Human IgM Fc5u alk phos Conjugated Antibody

ELISA Results of Purified Rabbit Anti-Human IgM Fc5µ Alkaline Phosphatase Conjugated Antibody tested against purified Human IgM Fc5µ protein. Each well was coated in duplicate with 1.0 µg of Human IgM Fc5µ (p/n orb346226). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using NPP Substrate.