Human IgM (Fc5u) Antibody Peroxidase Conjugated

• Catalog Number: orb347297
• Category: Antibodies
• Description: Human IgM (Fc5u) antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: 1.5 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgM and Human Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
• Immunogen: Human IgM Fc5µ fragment
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:15,000 - 1:60,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Anti-Human IgM (Fc5µ) Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Rabbit Anti-Human IgM (Fc5u) Antibody Peroxidase C
• Note: For research use only

Diagnostic accuracy of detecting IgG and IgG/M antibodies against natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) using HRP anti-human IgG (p/n orb347230), HRP anti-human IgM (p/n orb347297), and HRP Protein A. ELISA = enzyme-linked immunosorbent assay; NPV = negative predictive value; PPV = positive predictive value. Results are shown as percent, with confidence intervals in parentheses.

ELISA Results of Rabbit Anti-Human IgM F(c)5µ Antibody Peroxidase Conjugate tested against purified Human IgM F(c)5µ HRP. Each well was coated in duplicate with 1.0 µg of Human IgM F(c)5µ (p/n orb346226). The working dilution is 1:43000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n orb348651).