Human IgM (myeloma) Fc5u Antibody

SKU: orb346226

Description

Human IgM (myeloma) Fc5u Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Human IgM (myeloma) Fc5µ fragment has been tested in SDS-Page and can be used in Western Blotting and ELISA experiments as a control reagent.

Key Properties

• Source: Human
• Biological Origin: Human
• Isotype: IgM
• Purity: Human IgM (myeloma) Fc5µ fragment has been prepared from Human IgM myeloma protein by digestion with trypsin followed by column chromatography. Purity was assessed by SDS-PAGE and HPLC to be greater than 95%. A single precipitin arc was observed against anti-human IgM Fc5µ and anti-human serum when assayed by immuno-electrophoresis at a concentration of 20 mg/ml. No reaction was observed against anti-Trypsin, anti-human IgG F(ab')2, anti-human IgG F(c), anti-human Kappa or anti-human Lambda.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. Human IgM (myeloma) Fc5µ fragment is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.05% (w/v) Sodium Azide; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Concentration: 1.1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Human immunoglobulin M fragment; Human IgM Fc5µ

SDS PAGE Results of Human IgM Fc5µ. Lane 1: Human IgM Fc5µ reduced [5 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Human IgM Fc5µ non-reduced [5 µg]. 4-20% gel, Coomassie stained. Bands at expected MW.