Human IgG (H&L) Antibody Fluorescein Conjugated

• Catalog Number: orb347213
• Category: Antibodies
• Description: Human IgG (H&L) antibody (FITC)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Human IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 2.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG and Human Serum.
• Conjugation: FITC
• Biological Activity: 4.17
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Human IgG fluorescein conjugated Antibod
• Note: For research use only
• Application notes: Anti-Human IgG (H&L) Fluorescein has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of Goat Anti-Human IgG Fluorescein Conjugated Antibody. Human IgG (p/n orb346219) loaded: 1- 100 ng, 2- 33.33 ng, 3- 11.11 ng, 4- 3.70 ng, 5- 1.23 ng. Goat Anti-Human IgG Fluorescein Conjugated Antibody 1.0 µg/ml at RT for 60 mins. Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 30 mins at RT. Exposure: 0.2 secs. Fluorescein Filter.

Western Blot of Fluorescein conjugated Goat anti-Human IgG secondary antibody. Lane 1: Human IgG. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Human IgG. Other band(s): none.