Human IgG IgA IgM (H&L) Antibody Fluorescein Conjugated

• Catalog Number: orb347190
• Category: Antibodies
• Description: Human IgG IgA IgM (H&L) antibody (FITC)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: 2.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG, Human IgA and Human IgM. This reagent is suitable for the detection of all Human isotypes and chain combinations.
• Immunogen: Human IgG, IgA and IgM whole molecules
• Tested applications: DOT, FC, FLISA, IF, WB
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Application notes: Anti-Human IgG IgA IgM (H&L) Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Antibody Type: Secondary Antibody
• Biological Activity: 2.91
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Goat Anti-Human IgG IgA IgM Antibody fluorescein C
• Note: For research use only

Dot Blot of Goat anti-Human IgG IgA IgM Antibody Fluorescein Conjugated. Antigen: Row 1 - Human IgG Row 2 - Human IgA Row 3 - Human IgM. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Human IgG IgA IgM Antibody Fluorescein Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT.