Human IgG (H&L) Antibody Fluorescein Conjugated

• Catalog Number: orb346656
• Category: Antibodies
• Description: Human IgG (H&L) antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, FLISA, IF
• Reactivity: Human
• Isotype: IgG
• Immunogen: Human IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 10 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Human IgG and Human Serum.
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: rabbit anti-Human IgG Fluorescein Conjugated Antib
• Note: For research use only
• Application notes: Anti-Human IgG (H&L) Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

IgG Secretion. A) ARH-77, B) CCL-119, C) SA13. Both ARH-77 and SA13 cell lines display high fluorescence readouts while CCL-119 shows little fluorescence. Red circles correspond to MBs that contained cells and displayed high levels of immunoprecipitation. Light fluorescent spot in (B) is attributed to a reflection off an out of focus bubble on MB surface. By day 1 the fluorescent images showed that some wells took on a speckled appearance that continued to intensify over time (Fig. 1). By day 4 distinct differences could be discerned between the three cell lines. The fluorescence speckle pattern signifies detection of immunoprecipitation (IP) between secreted IgG and FITC labeled α-IgG added to the cell culture media.