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Human IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed

Catalog Number: orb347296

DispatchUsually dispatched within 5-10 working days
$ 430.00
Catalog Numberorb347296
CategoryAntibodies
DescriptionHuman IgG F(ab')2 antibody (Peroxidase)
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationHRP
ReactivityHuman
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
PurityAnti-Human IgG F(ab')2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.
ImmunogenHuman IgG F(ab')2 fragment
Tested applicationsELISA, IHC, WB
Dilution rangeELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Application notesAnti-Human IgG F(ab')2 Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,500 to 1:72,000 of the reconstitution concentration is suggested for this product. This product is also suitable for Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesrabbit Anti-Human IgG Fab2 Antibody peroxidase Con
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NoteFor research use only
Human IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed

ELISA results of purified Rabbit Anti-Human IgG F(ab')2 Peroxidase Conjugated Antibody tested against purified Human IgG F(ab')2. Each well was coated in duplicate with 1.0 µg of Human IgG F(ab')2 (blue line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.