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F(ab')2 Human IgG Fc Antibody Peroxidase Conjugated Pre-Adsorbed

Catalog Number: orb348189

DispatchUsually dispatched within 5-10 working days
$ 440.00
Catalog Numberorb348189
CategoryAntibodies
DescriptionF(ab')2 Human IgG F(c) antibody (Peroxidase)
ClonalityPolyclonal
Species/HostGoat
IsotypeIgG F(ab')2
ConjugationHRP
ReactivityHuman
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
PurityF(ab')2 Anti-Human IgG Fc was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab) or Bovine, Horse, Mouse and Rat Serum Proteins.
ImmunogenHuman IgG F(c) fragment
Tested applicationsELISA, IHC, WB
Dilution rangeELISA: 1:130,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Application notesF(ab')2 Anti-Human IgG F(c) Peroxidase Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesGoat F(ab')2 Anti-Human IgG F(c) Peroxidase Conjug
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NoteFor research use only
F(ab')2 Human IgG Fc Antibody Peroxidase Conjugated Pre-Adsorbed

ELISA results of purified F(ab')2 Goat anti-Human IgG F(c) Antibody Peroxidase Conjugated min x Bv, Hs, Ms, ant Rt serum proteins tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n orb346220). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n orb348651.