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F(ab')2 Human IgG IgA IgM (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

F(ab')2 Human IgG IgA IgM (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Catalog Number: orb348169

DispatchUsually dispatched within 5-10 working days
$ 440.00
Catalog Numberorb348169
CategoryAntibodies
DescriptionF(ab')2 Human IgG IgA IgM (H&L) antibody (Peroxidase)
ClonalityPolyclonal
Species/HostGoat
IsotypeIgG F(ab')2
ConjugationHRP
ReactivityHuman
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
PurityThis product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c) or Mouse Serum Proteins. This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.
ImmunogenHuman IgG, IgA, and IgM whole molecules
Tested applicationsELISA, IHC, WB
Dilution rangeELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Application notesF(ab')2 Anti-Human IgG IgA IgM (H&L) Peroxidase Antibody has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesGoat F(ab')2 Anti-Human IgG IgA IgM Peroxidase Con
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NoteFor research use only