HTR3A Antibody

SKU: orb1257401

Description

HTR3A Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-241 of human HTR3A (NP_000860.2).
• Target: HTR3A
• Molecular Weight: Observed: 55kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HTR3A, HTR3, 5HT3R, 5-HT-3, 5-HT3A, 5-HT3R

Western blot analysis of extracts of various cell lines, using HTR3A antibody (orb1257401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat brain using HTR3A antibody (orb1257401) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using HTR3A antibody (orb1257401) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

HTR3A

UniProt

P46098