HSP60 Antibody

• Catalog Number: orb749573
• Category: Antibodies
• Description: Recognizes a 60kDa protein, identified as the heat shock protein 60 (Hsp60). Its epitope is localized between amino acids 383-447 of human Hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial Hsp60, but also against its mammalian homolog.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Recombinant human protein was used as the immunogen for the anti-HSP60 antibody.
• UniProt ID: P10809
• Tested applications: IF, IHC-P, WB
• Dilution range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the anti-HSP60 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: SPM253
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the anti-HSP60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This anti-HSP60 antibody is available for research use only.
• Note: For research use only

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with anti-HSP60 antibody (clone SPM253).

Formalin-fixed, paraffin-embedded human pancreas stained with anti-HSP60 antibody (clone SPM253).

Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP60 antibody (clone SPM253, green) and Reddot nuclear stain (red).

Western blot testing of human HeLa lysate with HSP60 antibody (clone SPM253). Predicted molecular weight: ~60 kDa.