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HSP60 Antibody

Catalog Number: orb749573

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749573
CategoryAntibodies
DescriptionRecognizes a 60kDa protein, identified as the heat shock protein 60 (Hsp60). Its epitope is localized between amino acids 383-447 of human Hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial Hsp60, but also against its mammalian homolog.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
ImmunogenRecombinant human protein was used as the immunogen for the anti-HSP60 antibody.
UniProt IDP10809
Tested applicationsIF, IHC-P, WB
Dilution rangeImmunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application notesOptimal dilution of the anti-HSP60 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone NumberSPM253
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis anti-HSP60 antibody is available for research use only.
NoteFor research use only
HSP60 Antibody

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with anti-HSP60 antibody (clone SPM253).

HSP60 Antibody

Formalin-fixed, paraffin-embedded human pancreas stained with anti-HSP60 antibody (clone SPM253).

HSP60 Antibody

Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP60 antibody (clone SPM253, green) and Reddot nuclear stain (red).

HSP60 Antibody

Western blot testing of human HeLa lysate with HSP60 antibody (clone SPM253). Predicted molecular weight: ~60 kDa.

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