HSP60 Antibody / HSPD1

SKU: orb749574

Description

Recognizes a 60kDa protein, identified as the heat shock protein 60 (HSP60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog.

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the HSP60 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: GROEL/730
• Immunogen: Recombinant human protein was used as the immunogen for the HSP60 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human testicular carcinoma with HSP60 antibody (clone GROEL/730).

IHC testing of FFPE human pancreas with HSP60 antibody (clone GROEL/730).

IHC testing of FFPE human tonsil with HSP60 antibody (clone GROEL/730).

Immunofluorescent staining of MeOH-fixed human HeLa cells with HSP60 antibody (clone GROEL/730, green) and Reddot nuclear stain (red).

Western blot testing of human HeLa lysate with HSP60 antibody (clone GROEL/730). Predicted molecular weight: ~60 kDa.

Quick Database Links

UniProt

P10809