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    HSP60 Antibody

    Catalog Number: orb749575

    DispatchUsually dispatched within 5-10 working days
    $ 764.00
    Catalog Numberorb749575
    CategoryAntibodies
    DescriptionRecognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
    Species/HostMouse
    ClonalityMonoclonal
    Clone NumberHSPD1/780
    Tested applicationsIF, IHC-P, WB
    ReactivityHuman
    IsotypeMouse IgG1, kappa
    ImmunogenRecombinant human HSPD1 protein was used as the immunogen for the HSP60 antibody.
    Dilution rangeImmunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
    PurityProtein G affinity chromatography
    ConjugationUnconjugated
    Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
    Hazard InformationThis HSP60 antibody is available for research use only.
    UniProt IDP10809
    StorageStore the HSP60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
    Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
    NoteFor research use only
    Application notesOptimal dilution of the HSP60 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
    Expiration Date12 months from date of receipt.
    HSP60 Antibody

    IHC: Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with HSP60 antibody (clone HSPD1/780).

    HSP60 Antibody

    IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with HSP60 antibody (clone HSPD1/780).

    HSP60 Antibody

    IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with HSP60 antibody (clone HSPD1/780).

    HSP60 Antibody

    IHC: Formalin-fixed, paraffin-embedded human pancreas stained with HSP60 antibody (clone HSPD1/780).

    HSP60 Antibody

    Immunofluorescent staining of MeOH-fixed human HeLa cells with HSP60 antibody (clone HSPD1/780, green) and Reddot nuclear stain (red).

    HSP60 Antibody

    Immunofluorescent staining of MeOH-fixed human MCF7 cells with HSP60 antibody (clone HSPD1/780, green) and Reddot nuclear stain (red).

    HSP60 Antibody

    Western blot testing of human HeLa lysate with HSP60 antibody (clone HSPD1/780). Predicted molecular weight: ~60 kDa.

    HSP60 Antibody

    Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using HSP60 antibody (clone HSPD1/780). These results demonstrate the foremost specificity of the HSPD1/780 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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      FACS,  IF,  IHC-P,  WB

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      FACS,  IF,  IHC-P,  WB

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