HSP60 Antibody

SKU: orb1822503

Description

Mouse Anti-Human HSP60 Monoclonal IgG1

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB (1:4000), IHC (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Bacteria, Bovine, Canine, E. coli, Fish, Gallus, Guinea pig, H. pylori, Hamster, Human, Insect, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast
• Application Notes:
  0.25 µg/ml of SMC-111 was sufficient for detection of HSP60 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: LK2
• Immunogen: Recombinant human HSP60
• Target: HSP60
• Molecular Weight: 60kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

HSPD1, HSP60, 60 kDa heat shock protein, mitochondrial, Chaperonin 60, CPN60, HuCHA60, Heat shock protein family D member 1, GroEL homolog, mitochondrial, GROEL, HLD4, HSP 60, HSP65, SPG 13

Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.

Immunohistochemistry analysis using Mouse Anti-HSP60 Monoclonal Antibody, Clone LK2. Tissue: Kidney. Species: Human. Fixation: Formalin Fixed, Paraffin Embedded. Primary Antibody: Mouse Anti-HSP60 Monoclonal Antibody.

Western Blot analysis of Human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Hsp60 protein using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

HSP60

UniProt

P10809

NCBI Gene ID

3329

RefSeq (Protein):NP_002147.2