HSP60 Rabbit Polyclonal Antibody

SKU: orb10846

Description

HSP60 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HSP60 (501-573/573aa)
• Target: HSPD1
• Molecular Weight: 58 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

HSP 60; HSP-60; 60 kDa chaperonin; 60 kDa heat shock protein mitochondrial; 60kDa; cb863; Chaperonin 60; Chaperonin; Chaperonin, 60-KD; CPN 60; CPN60; fa04a05; fb22d10; fi27b05; GROEL; GroEL Homolog; GroEL, E. coli, homolog of; Heat shock 60kD protein 1(chaperonin); Heat shock 60kD protein 1 chaperonin; heat shock 60kDa protein 1(chaperonin); Heat Shock Protein 60; Heat shock protein 65; HLD4; Hsp 60; HSP 65; HSP65; HSPD 1; HSPD1; HuCHA60; id:ibd2197; Mitochondrial matrix protein P1; P60 lymphocyte protein; sb:cb144; Short heat shock protein 60 Hsp60s1; 60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; CH60_HUMAN.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HSP60) polyclonal Antibody, Unconjugated (orb10846) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP60) Polyclonal Antibody, Unconjugated (orb10846) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Adrenal gland (Mouse) Lysate at 40 ug, Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 4: Adrenal gland (Rat) Lysate at 40 ug, Lane 5: Jurkat (Human) Cell Lysate at 30 ug, Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 ug, Lane 7: HepG2 (Human) Cell Lysate at 30 ug, Lane 8: Hela (Human) Cell Lysate at 30 ug, Lane 9: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-HSP60 (orb10846) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated (orb10846) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated (orb10846) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

HSPD1

UniProt

P10809