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Catalog Number | orb248121 |
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Category | Antibodies |
Description | First described in relation to heat shock, HSPs are now known to be produced in response to additional stressful conditions including cold and UV light, as well as during wound healing. Heat shock protein 60 (HSP60) is responsible for protein transportation from the cytoplasm to the mitochondrial matrix and the subsequent refolding of the transported proteins. HSP60 also functions as a molecular chaperone, or chaperonin, assisting linear amino acid chains to fold into their three-dimensional structure. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | A recombinant protein fragment from human HSP60 was used as the immunogen for this antibody. |
Dilution range | Western blot: 1-3ug/ml,Immunohistochemistry (FFPE): 5-7ug/ml |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This HSP60 antibody is available for research use only. |
Entrez | 3329 |
Storage | Aliquot the HSP60 antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 1X PBS, pH 7.4 |
Note | For research use only |
Application notes | Provided assay concentrations are suggestions only, HSP60 antibody titration may be required for optimal results. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of human samples using HSP60 antibody (primary antibody dilution at: at 1 ug/ml)
Western blot analysis of mouse samples using HSP60 antibody (primary antibody dilution at: at 1 ug/ml)
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