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Hsd11b1 Antibody

SKU: orb1248849
KO/KD
Validated
KO/KD Validated

Description

Hsd11b1 Antibody

Research Area

Metabolism Research

Images & Validation

Tested ApplicationsELISA, KO/KD Validated, WB
ReactivityMouse
Predicted ReactivityRat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 32kDa band observed in Mouse Lung lysates (calculated MW of 32.4kDa according to NP_032314.2). Recommended concentration: 0.3-1µg/mL. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunizing peptide. However, it remained visible in a KO background.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-TARSEEGLQKVVSR
TargetHsd11b1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Hsd11b1, hydroxysteroid (11-beta) dehydrogenase 1, 11-DH, 11-beta-HSD1, HDL, HSD11, HSD11B, HSD11L, 11-beta-hydroxysteroid dehydrogenase 1, corticosteroid 11-beta-dehydrogenase isozyme 1, short chain dehydrogenase/reductase family 26C, member 1

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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