HSD11B1/HDL Antibody

SKU: orb18938

Description

Goat polyclonal antibody to HSD11B1

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 35kDa band observed in Human Liver lysates (calculated MW of 32.4kDa according to NP_005516.1 and NP_861420.1). Recommended concentration: 0.3-2 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HSD11B1 / HDL
• Protein Sequence: CTSYNMDRFINK
• Molecular Weight: 32.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti HSD11B1 antibody, anti HDL antibody, anti 11-DH antibody, anti HSD11L antibody, anti MGC13539 antibody, anti 11-beta-HSD1 antibody, anti hydroxysteroid (11-beta) dehydrogenase 1 antibody, anti corticosteroid 11-beta-dehydrogenase isozyme 1 antibody

orb18938 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

HSD11B1 / HDL

NCBI Gene ID

3290