HPRT1/HPRT Antibody

• Catalog Number: orb1537348
• Category: Antibodies
• Description: HPRT1/HPRT Antibody
• Target: HPRT1 / HPRT
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1.06 mg/ml
• Buffer/Preservatives: 1X PBS, pH 7, 0.025% ProClin 300, 20% Glycerol
• Purification: Immunoaffinity purified
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary.
• Tested applications: ICC, IF, IHC-P, WB
• Dilution range: ICC (1:100 - 1:1000), IF (1:100 - 1:1000), IHC-P (5 µg/ml), WB (1:500 - 1:3000)
• Application notes: Further information: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HPRT1, HGPRT, HGPRTase, HPRT
• Note: For research use only

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence of paraformaldehyde-fixed HeLa using HPRT antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate). A: Molt-4, B: Raji. 12% SDS PAGE. HPRT1 / HPRT antibody diluted at 1:1000.