HPRT1 Antibody (C-term)

• Catalog Number: orb1929842
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This HPRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-178 amino acids from the C-terminal region of human HPRT1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P00492
• MW: 24579 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB19377
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Hypoxanthine-guanine phosphoribosyltransferase, HG
• Note: For research use only
• NCBI: NP_000185.1

HPRT1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

HPRT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HPRT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of HPRT1 antibody (C-term) in Hela cell line lysates (35 ug/lane). HPRT1 (arrow) was detected using the purified Pab.

Western blot analysis of HPRT1 (arrow) using rabbit polyclonal HPRT1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HPRT1 gene.