HPRT1 Antibody (C-term)

SKU: orb1429593

Description

Rabbit polyclonal antibody to HPRT1.

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB19377
• Immunogen: Synthetic Peptide
• Target: HPRT1
• Molecular Weight: 24579

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT1, HPRT

Western blot analysis of HPRT1 antibody (C-term) in Hela cell line lysates (35 ug/lane). HPRT1 (arrow) was detected using the purified Pab.

HPRT1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

HPRT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HPRT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of HPRT1 (arrow) using rabbit polyclonal HPRT1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HPRT1 gene.

Quick Database Links

Gene Symbol

HPRT1

UniProt

P00492

RefSeq (Protein):NP_000185.1