HMGA1 Antibody

• Catalog Number: orb1260787
• Category: Antibodies
• Description: HMGA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGA1 (NP_665908.1).
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 16kDa
• Target: HMGA1
• UniProt ID: P17096
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: nonhistone chromosomal high-mobility group protein
• Note: For research use only
• Application notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of rat kidney, using HMGA1 antibody (orb1260787) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse brain using HMGA1 antibody (orb1260787) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using HMGA1 antibody (orb1260787) at dilution of 1:100 (40x lens).