HMGA1 Antibody

SKU: orb19076

Description

Goat polyclonal antibody to HMGA1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 4 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Placenta shows strong nuclear staining. Recommended concentration, 4-6ug/ml.WB: Preliminary experiments gave an approx 50kDa band in Human Testis and Ovary lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to isoform a (also called HMG-I; NP_665906.1; NP_665908.1; NP_665911.1) and 10.7kDa according to isoform b( (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1). The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HMGA1
• Protein Sequence: LASKQEKDGTEK
• Molecular Weight: 11.7; 10.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti high mobility group AT-hook 1 antibody, anti High mobility group AT-hook protein 1 antibody, anti High mobility group protein A1 antibody, anti High mobility group protein-R antibody

Immunohistochemical staining of Human Placenta using HMGA1 antibody

Immunohistochemical staining of Human Small Intestine using HMGA1 antibody

Quick Database Links

Gene Symbol

HMGA1

NCBI Gene ID

3159