HMGA1 Antibody (C-term)

SKU: orb1928108

Description

HMGA1 Antibody (C-term)

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution range: WB - 1:1000, IF - 1:10-50, FC - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Hamster, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 11676 Da

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

HMGIY

HMGA1 Antibody (C-term) western blot analysis in K562 cell line lysates (35 ug/lane).This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).

HMGA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-HMGA1 Antibody (C-term) at 1:1000 dilution + NCCIT whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).HMGA1 immunoreactivity is localized to Nucleus significantly.

Quick Database Links

UniProt

P17096