HMGA1 Antibody

• Catalog Number: orb1249518
• Category: Antibodies
• Description: HMGA1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SQPLASKQEKDGT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 24kDa band observed in Human Pancreas (calculated MW of 11.7kDa/10.7kDa according to NP_665906.1; NP_002122.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin and Placenta. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HMGA1
• UniProt ID: P17096
• NCBI: NP_002122.1, NP_665906.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HMGA1, high mobility group AT-hook 1, HMG-R, HMGA1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 24kDa band observed in Human Pancreas (calculated MW of 11.7kDa/10.7kDa according to NP_665906.1; NP_002122.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin and Placenta. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249518 (0.5 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.

orb1249518 (3.75 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249518 (3.75 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.