HMGA1 Antibody

SKU: orb1247391

Description

HMGA1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Testis and Ovary lysates after 0.3µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to isoform a (also called HMG-I; NP_665906.1; NP_665908.1; NP_665911.1) and 10.7kDa according to isoform b( (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1). The 50kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 4µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-LASKQEKDGTEK
• Target: HMGA1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HMGA1, high mobility group AT-hook 1 , HGNC:5010, HMG-R, HMGIY, MGC12816, MGC4242, MGC4854 , OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y, HMGA1A, OTTHUMP00000016223, OTTHUMP00000016224

Quick Database Links

Gene Symbol

HMGA1

UniProt

P17096

RefSeq (Protein):NP_002122.1, NP_665906.1, NP_665908.1, NP_665909.1, NP_665910.1, NP_665912.1