HLA-G Antibody (APC)

SKU: orb3130846

Description

HLA-G Antibody (APC)

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-5 μg/ml.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-G/11
• Immunogen: Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
• Target: HLA-G
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 0.1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-human HLA-G APC antibody (clone MEM-G/11) specificity verification by flow cytometry. Anti-human HLA-G APC (MEM-G/11) antibody (concentration in sample 5 μg/ml, red-filled histogram) binds specifically to HLA-G expressed on surface of HLA-G transfected LCL cells (upper panel), but not to surface of K562 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control APC antibody (MOPC-21) under same conditions (concentration in sample 5 μg/ml, black-dashed histogram).

Quick Database Links

Gene Symbol

HLA-G

UniProt

P17693

NCBI Gene ID

3135