HLA-G Antibody (APC)

SKU: orb44439

Description

Mouse Monoclonal to HLA-G.

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-5 μg/ml; positive control: JEG-3 human choriocarcinoma cell line.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-G/9
• Immunogen: Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
• Target: HLA-G
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 0.1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry analysis of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC (cat. no. orb44429).

Separation of LCL 721.221 HLA-G transfected cells stained using anti-human HLA-G (MEM-G/9) Alexa Fluor® 647 antibody antibody (10 µl reagent per million cells in 100 µl of cell suspension, red-filled) from LCL 721.221 HLA-G transfected cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 647 antibody (concentration in sample 1 µg/ml, same as CD135 APC concentration, black-dashed) in flow cytometry analysis (surface staining) of LCL 721.221 HLA-G transfected cells suspension.

Quick Database Links

Gene Symbol

HLA-G

UniProt

P17693

NCBI Gene ID

3135