HDAC1 Antibody

SKU: orb1248743

Description

HDAC1 Antibody

Images & Validation

• Tested Applications: ChIP, ELISA, IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Appox 55kDa band observed in Human Duodenum and Lymph Node lysates and approx 70kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 55.1kDa according to Human NP_004955.2 and Mouse NP_032254.1). The observed 70kDa band represents the sumoylated protein according to David et al, J Biol Chem. 2002 Jun 28;277(26):23658-63; PMID: 11960997. Recommended concentration: 0.1-2µg/mL.Immunofluorescence: Strong expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10µg/mL Chromatin Immunoprecipitation: A 3.5-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB07186 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KPEAKGVKEEVK
• Target: HDAC1
• Molecular Weight: Appox 55 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HDAC1, histone deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1 , reduced potassium dependency, yeast homolog-like 1

orb1248743 (0.5 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ChIP of 2 ug orb1248743 with 1 ug MCF7 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.

orb1248743 (0.1 ug/ml) staining of Human Duodenum (A) and Lymph Node (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248743 (0.1 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

orb1248743 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. The nuclear stain is DAPI.

Quick Database Links

Gene Symbol

HDAC1

UniProt

Q13547

RefSeq (Protein):NP_004955.2