Goat anti-Histone Deacetylase 1 Antibody

• Catalog Number: orb12395
• Category: Antibodies
• Description: Goat polyclonal antibody to HDAC1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IF, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:4000, WB: 0.1-2 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 55.1
• Target: Histone Deacetylase 1
• Entrez: 3065
• Protein Sequence: KPEAKGVKEEVK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti HDAC1 antibody, anti histone deacetylase 1 an
• Note: For research use only
• Application notes: WB: Appox 55kDa band observed in Human Duodenum and Lymph Node lysates and approx 70kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 55.1kDa according to Human NP_004955.2 and Mouse NP_032254.1). The observed 70kDa band represents. Notes: Immunofluorescence: Strong expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10µg/ml Chromatin Immunoprecipitation: A 3.5-fold increased presence of H3 onto the Human P21 l
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa lysate (35µg protein in RIPA buffer) using HDAC1 antibody.

Chromatin immunoprecipitation analysis of using HDAC1 antibody

Western blot analysis of Human Duodenum (A) and Lymph Node (B) lysate using HDAC1 antibody.

Western blot analysis of NIH3T3 lysate using HDAC1 antibody.