Histone Deacetylase 1 Antibody

SKU: orb12395

Description

Goat polyclonal antibody to HDAC1

Research Area

Cardiovascular Disease

Images & Validation

• Tested Applications: ChIP, ELISA, IF, WB
• Dilution Range: ELISA: 1:4000, WB: 0.1-2 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  WB: Appox 55kDa band observed in Human Duodenum and Lymph Node lysates and approx 70kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 55.1kDa according to Human NP_004955.2 and Mouse NP_032254.1). The observed 70kDa band represents. Notes: Immunofluorescence: Strong expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10µg/ml Chromatin Immunoprecipitation: A 3.5-fold increased presence of H3 onto the Human P21 l

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Histone Deacetylase 1
• Protein Sequence: KPEAKGVKEEVK
• Molecular Weight: 55.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti HDAC1 antibody, anti histone deacetylase 1 antibody, anti DKFZp686H12203 antibody, anti GON-10 antibody, anti HD1 antibody, anti RPD3 antibody, anti RPD3L1 antibody, anti reduced potassium dependency, yeast homolog-like 1 antibody

Western blot analysis of HeLa lysate (35µg protein in RIPA buffer) using HDAC1 antibody.

Chromatin immunoprecipitation analysis of using HDAC1 antibody

Western blot analysis of Human Duodenum (A) and Lymph Node (B) lysate using HDAC1 antibody.

Western blot analysis of NIH3T3 lysate using HDAC1 antibody.

Quick Database Links

Gene Symbol

Histone Deacetylase 1

NCBI Gene ID

3065