H2AFX Rabbit Polyclonal Antibody

SKU: orb583941

Description

Rabbit polyclonal antibody to H2AFX

Research Area

Epigenetics & Chromatin, Molecular Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Guinea pig, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human H2AFX
• Target: H2AFX
• Protein Sequence: Synthetic peptide located within the following region: ELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGKKATQASQE
• Molecular Weight: 16kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H2A.X, H2A/X, H2AFX

Immunohistochemistry with Tonsil tissue at an antibody concentration of 5 ug/ml using anti-H2AFX antibody (orb583941).

Sample Type: MCF7, Primary Antibody dilution: 4 ug/ml, Secondary Antibody: Anti-rabbit Alexa 546, Secondary Antibody dilution: 2 ug/ml, Gene Name: H2AFX.

WB Suggested Anti-H2AFX Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: NCI-H226 cell lysate. There is BioGPS gene expression data showing that H2AFX is expressed in NCIH226.

Quick Database Links

Gene Symbol

H2AFX

UniProt

P16104

RefSeq (Protein):NP_002096