Histone H2A.X (phospho Ser139) rabbit pAb Antibody

SKU: orb764201

Description

Histone H2A.X (phospho Ser139) rabbit pAb Antibody is a polyclonal, unconjugated rabbit antibody. It is suitable for detection in Hamster, Human, Mouse, Rat samples. The antibody is generated using a synthesized peptide derived from human Histone H2A.X around the phosphorylation site of Ser139 AA range 94-143 and can be used for ELISA, IF, IHC, WB applications. It is supplied as an IgG isotype antibody in PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Hamster, Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H2A.X around the phosphorylation site of Ser139. AA range:94-143
• Molecular Weight: 15 19
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-H2AFX antibody, anti-H2AX antibody, anti-Histone H2A.x antibody, anti-H2a/x antibody

Quick Database Links

UniProt

P16104

Kimoto, Saki et al. Nacre extract derived from Pinctada fucata mitigates skeletal muscle aging by suppressing senescence-associated inflammation and preserving contractile features in naturally aged mice and C2C12 myotubes Asian Pacific Journal of Tropical Biomedicine, (2026)

PubMed:

Applications

IF

Reactivity

Mouse

Momoko Kawaminami et al. Nacre extract attenuates age-related functional and tissue alterations under post-onset intervention conditions in two murine aging models Biogerontology, 27, 114 (2026) (2026)

PubMed: 42289617

Applications

IF, IHC

Reactivity

Mouse